abstract：:We tested the hypothesis that the early action potential shortening induced by hypoxia in perfused hearts is attributable to chloride currents activated or modulated by endogenous catecholamine release. Rabbit hearts perfused at 33 degrees C and paced at 2.5-2.8 Hz were used for membrane potential recordings with micr...

journal_title：Journal of molecular and cellular cardiology

authors： Ruiz Petrich E,Ponce Zumino A,Schanne OF

更新日期：1996-02-01 00:00:00

abstract：:We determined whether the dilated cardiomyopathy which develops between 30 and 140 days of age in the Syrian hamster strain MS200, before the onset of cardiac hypertrophy and failure, is associated with alterations in both the action potential (AP) and the Ca(2+)-independent transient outward current, Ito1. AP was rec...

journal_title：Journal of molecular and cellular cardiology

authors： Thuringer D,Coulombe A,Deroubaix E,Coraboeuf E,Mercadier JJ

更新日期：1996-02-01 00:00:00

abstract：:The Na+/Ca2+ exchanger plays an important role in the maintenance of calcium homeostasis in the heart. Therefore, factors which regulate the exchanger have a significant impact on cardiac function. Previously, we showed that the non-hydrolysable GTP analog, 5'guanylyl imidodiphosphate [Gpp(NH)p], stimulates Na+/Ca2+ e...

journal_title：Journal of molecular and cellular cardiology

authors： Ballard C,Schaffer S

更新日期：1996-01-01 00:00:00

abstract：:The incidence of ventricular arrhythmias is higher in failing hearts than in control hearts, especially during acute ischemia. Electrophysiological and extracellular ionic changes during acute ischemia in normal and failing rabbit myocardium were assessed. Heart failure was induced in rabbits by combined volume and pr...

journal_title：Journal of molecular and cellular cardiology

authors： Vermeulen JT,Tan HL,Rademaker H,Schumacher CA,Loh P,Opthof T,Coronel R,Janse MJ

更新日期：1996-01-01 00:00:00

abstract：:RP58866 (1-[-2-(3,4-dihydro-2H-1-benzopyran-4-yl)ethyl]-4- (3,4-dimethoxyphenyl)-piperidine), a specific blocker of the inwardly rectifying K+ current (IK1), is an extremely effective antiarrhythmic agent in rat, rabbit and primate (marmoset) isolated hearts in the settings of acute ischaemia and reperfusion (Rees and...

journal_title：Journal of molecular and cellular cardiology

authors： Rees SA,Curtis MJ

更新日期：1995-12-01 00:00:00

abstract：:The effects of cyclopiazonic acid, a specific inhibitor of the sarcoplasmic reticulum Ca(2+)-ATPase, on membrane currents and contraction were investigated under voltage clamped conditions on frog atrial trabeculae using the double mannitol-gap technique. In ringer solution, cyclopiazonic acid (10 microM) decreased th...

journal_title：Journal of molecular and cellular cardiology

authors： Badaoui A,Huchet-Cadiou C,Léoty C

更新日期：1995-11-01 00:00:00

abstract：:The aim of this study was: (1) to elucidate in more detail the relationship between stress protein expression and brief periods of ischaemia and reperfusion, such as occur during early (classical) ischaemic preconditioning (PC) in the rabbit myocardium; (2) to determine whether stress protein expression is affected by...

journal_title：Journal of molecular and cellular cardiology

authors： Heads RJ,Latchman DS,Yellon DM

更新日期：1995-10-01 00:00:00

abstract：:We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals we...

journal_title：Journal of molecular and cellular cardiology

authors： Eschenhagen T,Laufs U,Schmitz W,Scholz H,Warnholtz A,Weil J,Schäfer HJ

更新日期：1995-10-01 00:00:00

abstract：:Cardiac hypertrophy is characterized, among others, by the molecular events which selectively activate the expression of genes for contractile proteins within individual myocardial cells. As such, myosin light chain 2 (MLC-2), which is upregulated in the hypertrophic state in both rat and human, serves as a marker for...

journal_title：Journal of molecular and cellular cardiology

authors： Doud SK,Pan LX,Carleton S,Marmorstein S,Siddiqui MA

更新日期：1995-10-01 00:00:00

abstract：:Type V and VI adenylyl cyclase mRNAs are the two main cyclase isoforms expressed in the mammalian heart. A recent report has shown that their expression is differentially regulated during ontogenic development, but the accumulation of the two mRNA species and their concentration ratio have not been determined. We thus...

journal_title：Journal of molecular and cellular cardiology

authors： Espinasse I,Iourgenko V,Defer N,Samson F,Hanoune J,Mercadier JJ

更新日期：1995-09-01 00:00:00

abstract：:Micromolar concentrations of lipoic acid racemate added to a working rat heart during hypoxia have been previously found to improve aortic flow during subsequent reoxygenation. Since the R-form represents the naturally occurring form of lipoic acid, and the S-form does not reveal a positive influence on ATP synthesis ...

journal_title：Journal of molecular and cellular cardiology

authors： Zimmer G,Beikler TK,Schneider M,Ibel J,Tritschler H,Ulrich H

更新日期：1995-09-01 00:00:00

abstract：:We made acute cardiac failure in excised cross-circulated canine hearts by a new coronary perfusion protocol consisting of Ca2+ free Tyrode perfusion for the first 10 min, high Ca2+ (16 mmol/l) Tyrode perfusion for the next 5 min, and normal Tyrode perfusion for the last 5 min interrupting blood cross circulation. Aft...

journal_title：Journal of molecular and cellular cardiology

authors： Takaki M,Zhao DD,Zhao LY,Araki J,Mori M,Suga H

更新日期：1995-09-01 00:00:00

abstract：:To determine the regulatory role of prolyl hydroxylation in intracellular cardiac procollagen turnover, we examined the effects of prolyl 4-hydroxylase inhibitors (alpha, alpha-dipyridil, 3,4-dihydroxybenzoic acid ethyl ester, pyridine 2,4-dicarboxylic acid ethyl ester) and ascorbic acid on procollagen metabolism by c...

journal_title：Journal of molecular and cellular cardiology

authors： Eleftheriades EG,Ferguson AG,Spragia ML,Samarel AM

更新日期：1995-08-01 00:00:00

abstract：:In susceptible DBA/2 mice coxsackievirus B 3-induced myocarditis leads to inflammatory and necrotic lesions in the myocardium 7-10 days after virus inoculation. The purpose of this study was to determine whether hemodynamic changes occur in murine coxsackievirus B 3 myocarditis and whether they are correlated to histo...

journal_title：Journal of molecular and cellular cardiology

authors： Herzum M,Weller R,Jomaa H,Wietrzychowski F,Pankuweit S,Mahr P,Maisch B

更新日期：1995-08-01 00:00:00

abstract：:The dynamics of the changes in myocardial phosphorylated compound contents (inorganic phosphate: Pi; phosphocreatine: PCr; ATP) induced by 10(-6)M isoprenaline administration was studied, using 31P-NMR spectroscopy, in hearts isolated from rats adapted for three weeks to normobaric hypoxia (10% of oxygen). When compar...

journal_title：Journal of molecular and cellular cardiology

authors： Novel-Chaté V,Aussedat J,Saks VA,Rossi A

更新日期：1995-08-01 00:00:00

abstract：:Fibrillar collagens, essential for maintaining the structural integrity of the myocardium, are degraded by matrix metalloproteinase (MMP-1). In other tissues collagenolysis is an important component of wound healing. Here we examined collagen degradation in the myocardium after infarction. Collagenase activity, measur...

journal_title：Journal of molecular and cellular cardiology

authors： Cleutjens JP,Kandala JC,Guarda E,Guntaka RV,Weber KT

更新日期：1995-06-01 00:00:00

abstract：:Cardiac myocytes have a high affinity. Mg(2+)-dependent, electrogenic, Na-inositol co-transporter on the plasma or sarcolemma (SL) membrane (Rubin and Hale, 1993). The ion specificity and stoichiometry of this transport process is unknown. Using bovine cardiac SL vesicles, we have determined that transmembrane movemen...

journal_title：Journal of molecular and cellular cardiology

authors： Hale CC,Rubin LJ

更新日期：1995-05-01 00:00:00

abstract：:Diacylglycerol analogs and phorbol esters are used as protein kinase C (PKC) activators to investigate the effect of PKC on L-type calcium current [ICa(L)] in cardiomyocytes. 1-2-dioctanoyl-sn-glycerol (diC8) is a potent analog of diacylglycerol (DAG) which produces positive inotropic effects in guinea-pig atria cardi...

journal_title：Journal of molecular and cellular cardiology

authors： Conforti L,Sumii K,Sperelakis N

更新日期：1995-05-01 00:00:00

abstract：:Fibronectin (FN) is a dimeric glycoprotein found in the extracellular matrix of most tissues that serves as a bridge between cells and the interstitial collagen meshwork and influences diverse processes including cell growth, adhesion, migration, and wound repair. Multiple FN forms arise by the alternative splicing of...

journal_title：Journal of molecular and cellular cardiology

authors： Farhadian F,Contard F,Corbier A,Barrieux A,Rappaport L,Samuel JL

更新日期：1995-04-01 00:00:00

abstract：:The effects of preventing oxidative phosphorylation on pHi were compared in papillary muscles from right ventricles of normal and pressure-overloaded ferret hearts. Hypertrophy was induced by pulmonary artery clipping for 30-45 days. pHi was recorded with pH-sensitive microelectrodes. Resting pHi and the relationship ...

journal_title：Journal of molecular and cellular cardiology

authors： Do E,Baudet S,Gow IF,Ellis D,Noireaud J

更新日期：1995-03-01 00:00:00

abstract：:The topic of this review is the lipidic part of the sarcolemma, the plasma membrane of the myocardial cell, and its role in (dis)function of the cardiomyocyte. First the isolation of the sarcolemma and its lipid composition are discussed. These phospholipids are not randomly distributed over the two monolayers of the ...

journal_title：Journal of molecular and cellular cardiology

authors： Post JA,Verkleij AJ,Langer GA

更新日期：1995-02-01 00:00:00

abstract：:Lidocaine is a Na+ channel blocker that is highly effective for the treatment of ventricular tachyarrhythmias, but is largely ineffective against atrial arrhythmias. If is not known if this differential efficacy is the result of differences in lidocaine inhibition of atrial v ventricular Na+ channels. The purpose of t...

journal_title：Journal of molecular and cellular cardiology

authors： Furukawa T,Koumi S,Sakakibara Y,Singer DH,Jia H,Arentzen CE,Backer CL,Wasserstrom JA

更新日期：1995-02-01 00:00:00

abstract：:The incidence of mortality from cardiovascular diseases in higher in diabetic patients. The cause of this accelerated cardiovascular disease is multifactorial and, although atherosclerotic cardiovascular disease in association with well-defined risk factors has an influence on morbidity and mortality in diabetics, myo...

journal_title：Journal of molecular and cellular cardiology

authors： Rodrigues B,Cam MC,McNeill JH

更新日期：1995-01-01 00:00:00

abstract：:Effects of hypoxia-reoxygenation (H-R) on myocytes isolated from 10 week hypertrophied and sham control rat hearts were studied. Myocyte hypertrophy was indicated by an increase in cell size. Superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) enzyme activities were significantly higher and lipid peroxidatio...

journal_title：Journal of molecular and cellular cardiology

authors： Kirshenbaum LA,Hill M,Singal PK

更新日期：1995-01-01 00:00:00

abstract：:Studies of hemodynamics and intrinsic left ventricular myocardial function are carried out to investigate the transition from stable hypertrophy to cardiac decompensation in the aging (18-24 months) spontaneously hypertensive rat (SHR). Echocardiographic data in awake animals demonstrate increased end-diastolic and en...

journal_title：Journal of molecular and cellular cardiology

authors： Bing OH,Brooks WW,Robinson KG,Slawsky MT,Hayes JA,Litwin SE,Sen S,Conrad CH

更新日期：1995-01-01 00:00:00

abstract：:We have studied the effects of pressure and volume overload as well as of norepinephrine (NE) alone and in combination on the expression of the proto-oncogenes c-fos and c-myc in isolated perfused working rat hearts. Using specific cDNA clones, the mRNAs of c-fos and c-myc were measured by Northern blots and quantifie...

journal_title：Journal of molecular and cellular cardiology

authors： Kolbeck-Rühmkorff C,Zimmer HG

更新日期：1995-01-01 00:00:00

abstract：:Insulin increases the synthesis of mitochondrial proteins in the isolated perfused heart and total cell protein synthesis in neonatal cardiac myocytes. Since carnitine-dependent fatty acid oxidation is modulated by insulin in a variety of tissues, the effects of 1.7 microM insulin on the mitochondrial enzyme(s), carni...

journal_title：Journal of molecular and cellular cardiology

authors： Hudson EK,Liu MH,Buja LM,McMillin JB

更新日期：1995-01-01 00:00:00

abstract：:Vascular tone is regulated by a variety of neurotransmitters, vasoactive hormones and autacoids, and vasoactive drugs. These actions are mediated, at least in part, by actions on the membrane ion channels, exerted either directly or indirectly. In this article, we described evidence that four different protein kinase ...

journal_title：Journal of molecular and cellular cardiology

authors： Xiong Z,Sperelakis N

更新日期：1995-01-01 00:00:00

abstract：:In this study, calcium overload during reperfusion and the severity of morphologically evident ischemic myocardial injury were compared in hypertrophied and normal hearts. Hypertrophied hearts isolated from rats where a clip had been placed on the proximal thoracic aorta for 6 weeks were compared to those from sham-op...

journal_title：Journal of molecular and cellular cardiology

authors： Allard MF,Flint JD,English JC,Henning SL,Salamanca MC,Kamimura CT,English DR

更新日期：1994-12-01 00:00:00

abstract：:Insulin-like growth factor I (IGF I) is an important mitogen for vascular smooth muscle cells (VSMC). The IGF I receptor (IGF IR) is a heterotetramer composed of two cross-linked extracellular alpha-chains and two membrane-spanning beta-chains that contain a tyrosine-kinase domain. It has a high degree of sequence sim...

journal_title：Journal of molecular and cellular cardiology

authors： Delafontaine P,Ku L,Ververis JJ,Cohen C,Runge MS,Alexander RW

更新日期：1994-12-01 00:00:00

abstract：:Previous animal experiments indicated that the effects of catecholamines on myocardial function and subcellular systems vary considerably depending on the species and type of myocardium investigated. In the present study, we used isometric force and heat measurements to investigate the influence of isoproterenol on en...

journal_title：Journal of molecular and cellular cardiology

authors： Hasenfuss G,Mulieri LA,Leavitt BJ,Alpert NR

更新日期：1994-11-01 00:00:00

abstract：:Our own previous ultrastructural studies in human hearts with dilated cardiomyopathy and heart failure showed sarcomeric and cytoskeletal disarrangement. On the basis of these findings we tested the hypothesis that in cardiomyopathic failing hearts not only the sarcomere structure but also the organization and the amo...

journal_title：Journal of molecular and cellular cardiology

authors： Hein S,Scholz D,Fujitani N,Rennollet H,Brand T,Friedl A,Schaper J

更新日期：1994-10-01 00:00:00

abstract：:The effects of preconditioning, adenosine and dipyridamole in protecting the systolic and diastolic alterations of myocardial stunning in rabbit hearts were studied. Isovolumic left ventricular developed pressure (LVDP), and end diastolic pressure (LVEDP) were measured. The time constant of relaxation (T) was calculat...

journal_title：Journal of molecular and cellular cardiology

authors： Mosca SM,Gelpi RJ,Cingolani HE

更新日期：1994-10-01 00:00:00

abstract：:Doxorubicin (Dox) is a widely used antineoplastic agent. Irreversible cardiomyopathy is a serious and dose-limiting side effect after chronic administration. The iron chelating bispiperazinedione ICRF-187 is currently the only drug which affords protection against Dox-induced cardiotoxicity. To compare the protective ...

journal_title：Journal of molecular and cellular cardiology

authors： Voest EE,van Acker SA,van der Vijgh WJ,van Asbeck BS,Bast A

更新日期：1994-09-01 00:00:00

abstract：:This study was undertaken to evaluate the direct cardioprotective effect of trimetazidine (TMZ), an anti-anginal drug devoid of haemodynamic action, on isolated myocytes. Cultured rat ventricular myocytes were treated with the drug 16 h and 1 h before the experiments. The drug-treated cells and control cells were plac...

journal_title：Journal of molecular and cellular cardiology

authors： Fantini E,Demaison L,Sentex E,Grynberg A,Athias P

更新日期：1994-08-01 00:00:00

abstract：:To elucidate the role of bicarbonate-dependent mechanisms and Na+/H+ exchange in maintenance of physiological intracellular pH (pHi) under various steady-state conditions, phosphorus NMR spectra were taken in isovolumically-contracting, perfused ferret hearts. Switching the perfusate from HCO3-/CO2 to HEPES buffer sig...

journal_title：Journal of molecular and cellular cardiology

authors： Kusuoka H,Marban E,Cingolani HE

更新日期：1994-07-01 00:00:00

abstract：:Thromboxane A2 (TXA2) and prostaglandin H2 (PGH2) induce platelet aggregation and are potent vasoconstrictors, and they have been implicated in coronary vasospasm and myocardial infarction. The TXA2 mimetic [1S-(1 alpha, 2 beta (5Z), 3 alpha (1E,3S*), 4 alpha)]-7-[3-(3-hydroxy-4-(4'- iodophenoxy)-1-butenyl)-7-oxabicyc...

journal_title：Journal of molecular and cellular cardiology

authors： Bowling N,Dubé GP,Kurtz WL,Brune KA,Saussy DL Jr,Dorn GW 2nd,Mais DE

更新日期：1994-07-01 00:00:00

abstract：:Heat shock protein 70 (HSP70) has been shown to play a fundamental role in the induction of thermotolerance in many biological systems. Elevated synthesis of HSP70 in response to diverse stresses such as heat, anoxia, ischaemia, ethanol and heavy metals has been correlated with protection against subsequent more sever...

journal_title：Journal of molecular and cellular cardiology

authors： Heads RJ,Latchman DS,Yellon DM

更新日期：1994-06-01 00:00:00

abstract：:Free radical generating systems produce a sequence of changes in the action potentials of isolated cardiac myocytes. Efforts to understand the nature of the alterations in ionic currents have yielded mixed results. The use of patch-pipettes for voltage-clamp experiments and manipulation of pipette solution composition...

journal_title：Journal of molecular and cellular cardiology

authors： Barrington PL

更新日期：1994-05-01 00:00:00

abstract：:We used three interventions to test critically the theory that ischemic preconditioning is the result of translocation of cytosolic protein kinase C (PKC) into the membranes where it can be activated. If that theory were true then kinase activity should not be necessary during the preconditioning ischemia and thus blo...

journal_title：Journal of molecular and cellular cardiology

authors： Liu Y,Ytrehus K,Downey JM

更新日期：1994-05-01 00:00:00